LI qinfan

LI Qinfan

Ph. D., Professor.

Email: liqf1131@163.com

Department of Clinical Veterinary Medicine,

College of Veterinary Medicine,

Northwest A&F University,

No.3 Taicheng Road, Yangling, Shaanxi, China, 712100

Education:

1/9/1987-1/7/1992 B.S. in Veterinary Medicine, Northwest Agricultural University, Shaanxi, China

1/9/2002-1/7/2005 M.S. in Clinical Veterinary Science, Northwest A&F University, Shaanxi, China

1/9/2001-1/72005 Ph.D. in Clinical Veterinary Science, Northwest A&F University, Shaanxi, China

1/10/2006-1/10/2007 Visiting Institute for Medical Chemistry, DNS, University for Veterinary Medicine, Vienna, Austria

Area of Research:

Animal disease and Toxicology, focuses on the structure and function of  Capra hircus  lysosoma α-mannosidase ( ch LAM) and  Capra hircus golgi α-mannosidaseⅡ ( ch GMII), including cloning and expressing the gene, studying the character, determining active center and building the 3D structure of  ch LAM and  ch GMII. Elucidate the molecular mechanism about SW inhibits α-mannosidase.

Research Grants

1/2019-12/2022 The replacement of domain binding swainsonine and its functional verification in  Capra hircus lysosomal α-mannosidase, National Nature Science Foundation of China (No. 31860724)

1/2017-12/2019 Research and demonstration of key technologies for prevention and control of mastitis and endometritis, Key R & D projects of Shaanxi Province-agricultural field, China (No. 2017ZDXM-NY-079

1/2014-12/2016 The development and application of the enzyme preparation of swainsonine poisoning in sheep, Shaanxi science and technology project, China (No. 2014K01-17-02)

1/2008-12/2010 Degradation of toxic alkaloid TMPD from  Oxytropis glacialis by bacteria, Shaanxi science and technology project, China (No. 2008K02-06)

Selected Publications (†indicates contributed equally, *indicates corresponding author)

[1] Li Qinfan, Wang Yingzi, Rudolf Moldzio, Lin Weimin, Wolf-Dieter Rausch*. Swainsonine as a lysosomal toxin affects dopaminergic neurons，Journal of Neural Transmission，2012,119: 1483-1490.

[2] Li Qinfan†, Zhang Lihui†, Ma Zhi, Kong Xiangya, Wang Fangfang, Zhang Hong, Wang Yonghua*. Probing structural features and binding mode of 3-Arylpyrimidin-2,4-diones within housefly γ-Aminobutyric acid (GABA) receptor. International Journal of Molecular Sciences，2011,12(9): 6293-63111.

[3] Li Qinfan†, Kong Xiangya†, Xiao Zhengtao, Zhang Lihui, Wang Fangfang, Zhang Hong, Li Yan, Wang Yonghua*. Structural determinants of imidacloprid-based nicotinic acetylcholine receptor inhibitors identified using 3D-QSAR,docking and molecular dynamics, J Mol Model. 2012, 18: 2279–2289.

[4] Li Qinfan, Hao Caiju, Xu Yongping, Liang Jie, Yang Kai, Cui Zhonghua. Identification of a new Locoweed ( Oxytropis serioopetala ) and its clinical and pathological features in poisoned rabbits. J Vet Med Sci，2012,8：989-993.

[5] Li Qinfan†, Fang Yupeng†, Li Xiuxiu, Zhang Hong, Liu Mengmeng, Yang Huibin, Kang Zhuo, Li Yan, Wang Yonghua*. Mechanism of the plant cytochrome P450 for herbicide resistance: a modelling study，Journal of Enzyme Inhibition and Medicinal Chemistry, 2013,28(6):1182-1191.

[6] Kong Xiangya, Zhang Jiangye, Wu Ying, Li Jianfei，Li Qinfan*. Molecular characterization of  Capra hircus lysosomal α-mannosidase and potential mutant site for the therapy of locoweed poisoning，Acta Biochim Pol. 2014, 61(1): 77-84.

[7] Li Jianfei, Zhang Jiangye, Lai Bi, Zhao Ying, Li Qinfan*. Cloning, expression, and characterization of  Capra hircus Golgi α-Mannosidase II. Appl Biochem Biotechnol, 2015, 177(6): 1241-1251.

[8] Yang Kai, Liang Jie, Li Qinfan*, Kong Xiangya, Chen Rui, Jin Yimin. Cladosporium cladosporioides XJ-AC03, an aconitine-producing endophytic fungus isolated from  Aconitum leucostomum . World Journal of Microbiology and Biotechnology. 2013, 29: 933–938 .

[9] Liu Xiaoqiang, Liu Haixia, Wang Le, Peng Qian, Li Yinqian, Zhou Hongchao, Li Qinfan*. Molecular characterization of extended-spectrum β-lactamase- producing multidrug resistant  Escherichia coli from swine in northwest China. Front Microbiol. 2018, 9:1756.doi:10. 3389

[10] Wang Yan, Zhai A’guan, Zhang Yanqi, Qiu Kai, Wang Jianhua, Li Qinfan*. by Swainsonine of Degradation the NADP-Dependent Alcohol Dehydrogenase A1R6C3 in  Arthrobacter sp.HW08. Toxins, 2016, 8(5): 145.

[11] Xu Xingang†, Xu Ying†, Zhang Qi, Yang Feng, Yin Zheng, Wang Lixiang, Li Qinfan*. Porcine epidemic diarrhea virus infections induce apoptosis in Vero cells via a reactive oxygen species (ROS)/p53, but not p38 MAPK and SAPK/JNK signalling pathways. Vet Microbiol. 2019 May; 232:1-12. doi: 10.1016/j.vetmic.2019.03.028. Epub 2019 Mar 30.

Awards

The Second Prize of Shaanxi Science and Technology Progress Award (R2), 2007, Shaanxi Provincial Government, China.