刘军
一、个人基本信息
刘军,男,汉族,1981年10月出生,黑龙江人,教授,博士生导师。现任永乐高ylg888888临床兽医系教授。主持科技创新2030-农业生物育种重大项目“优质高产抗病乳用羊新品种设计与培育”,以及国家自然科学基金项目等国家和省部级项目6项,参与转基因重大专项“抗病转基因牛新品种培育”项目的研究,发表SCI 论文 20 余篇,获得国家技术发明二等奖 1 项(第六完成人),陕西省科学技术一等奖 2 项(分别为第三和第五完成人)。
研究方向动物胚胎工程。主要开展以下研究:(1)研究牛羊早期胚胎发育调控机制,探索提高体细胞克隆效率的新方法;(2)利用动物克隆和基因编辑技术建立高效奶山羊乳腺生物反应器平台,表达重要营养和药用蛋白;(3)创新胚胎体外生产技术体系,快速扩繁顶级牛、羊良种。
二、学习和工作经历
2000年09月-2004年06月:永乐高ylg888888动物医学专业,获学士学位。
2004年09月-2007年06月:永乐高ylg888888临床兽医学专业,获硕士学位。
2007年09月-2011年06月:永乐高ylg888888临床兽医学专业,获博士学位。
2011年7月-2013年6月:西北农林科技大学生命科学院发育生物学博士后。
2013年6月-2013年12月:永乐高ylg888888讲师。
2014年1月-2020年12月:永乐高ylg888888副教授。
2018年7月-2019年7月:美国斯坦福大学医学院高级访问学者。
2021年5月-2022年6月:科技部中国农村技术开发中心项目专员。
2021年1月-今:永乐高ylg888888教授。
三、讲授课程
讲授《畜牧学概论》等本科生课程,指导本科临床实习和畜牧学概论实习,参与《动物胚胎工程》等MOOC线上课程建设。讲授《动物克隆与转基因技术》、《发育生物学》、《实验动物学》研究生课程。
四、代表性学术成果
1. Miaomiao Jin#, Lu Zhao#, Hanwen Yang, Jianglin Zhao, Hongwei Ma, Yanzhi Chen, Jingcheng zhang, Yan Luo*, Yong Zhang*, and Jun Liu*. A long non-coding RNA essential for early embryonic development improves SCNT efficiency in goats. Reproduction 2023; DOI: https://doi.org/10.1530/REP-23-0053.(通讯作者, 中科院二区, IF3.8)
2. Yupei Yao#, Yujie Tang, Haoqiang Qin, Ru Meng, Chengtu Zhang, Yingbing Zhang, Ying Yang, Peipei Qiao, Jun Liu*, Jianmin Su*. Zinc supplementation promotes oocyte maturation and subsequent embryonic development in sheep. Theriogenology 2023; 206:161-169. (通讯作者, 中科院二区TOP, IF2.8).
3. Min Huang, Xuyang Cao, Qifu He, Hanwen Yang, Yanzhi Chen, Jinglin Zhao, Hongwei Ma, Jian Kang, Jun Liu*, Fusheng Quang*. Alkaline semen diluent combined with R848 for separation and enrichment of dairy goat X-sperm. Journal of Dairy Science 2022;105(12):10020-10032.(通讯作者, 中科院一区TOP, IF3.5)
4. Rui Cheng #, Xiaoman Zheng #, Yingmei Wang, Xing Ma, Xin Liu, Wenjun Xu, Mengyun Wang, Yuanpeng Gao, Xupeng Xing, Chuan Zhou, Hongzheng Sun, Zekun Guo, Fusheng Quan, Jun Liu*, Song Hua*, Yongsheng Wang*, Yong Zhang *, Xu Liu *. Modification of alternative splicing in bovine somatic cell nuclear transfer embryos using engineered CRISPR-Cas13d. Science China-Life Sciences 2022; 65(11):2257-2268(通讯作者, 中科院一区TOP, IF10.372)
5. Qing Zhang#, Wenjing Li, Pei Feng, Yayi Liu, Peng Meng, Bo Chu, Jianglin Zhao, Yanxue Li, Yong Zhang*, Jun Liu*. Lnc5926 is essential for early embryonic development in goats through regulation of ZSCAN4 and EIF1AX. Theriogenology 2022; 180(1):87-93.(通讯作者, 中科院二区TOP, IF2.8)
6. Jiaxin Feng#, Qianwen Wang, Wei Yang, Jun Liu*, Ming-Qing Gao*. Omega-3 polyunsaturated fatty acids ameliorated inflammatory response of mammary epithelial cells and mammary gland induced by lipopolysaccharide. Acta Biochimica et Biophysica Sinica 2021; 53(9): 1142-1153.(通讯作者, 中科院二区, IF3.7)
7. Chengquan Han#, Chenchen Cui, Xupeng Xing, Zhenzhen Lu, Jingcheng Zhang, Jun Liu*, Yong Zhang*. Functions of intrinsic disorder in proteins involved in DNA demethylation during pre-implantation embryonic development. International Journal of Biological Macromolecules 2019; 136:962-979.(通讯作者, 中科院一区TOP, IF8.2)
8. Chengquan Han#, Ruizhi Deng, Tingchao Mao, Yan Luo, Biao Wei, Peng Meng, Lu Zhao, Qing Zhang, Fusheng Quan, Jun Liu*, Yong Zhang*. Overexpression of Tet3 in donor cells enhances goat somatic cell nuclear transfer efficiency. FEBS Journal 2018; 285(14):2708-2723.(通讯作者, 中科院二区, IF5.542)
9. Ruizhi Deng#, Chengquan Han, Lu Zhao, Qing Zhang, Beifen Yan, Rui Cheng, Biao Wei, Peng Meng, Tingchao Mao, Yong Zhang*, Jun Liu*. Identification and characterization of ERV transcripts in goat embryos. Reproduction 2018; 157(1):111-122.(通讯作者, 中科院二区, IF3.8)
10. Tingchao Mao#, Chengquan Han, Biao Wei, Lu Zhao, Qing Zhang, Ruizhi Deng, Jun Liu*, Yan Luo*, Yong Zhang*. Protective Effects of Quercetin Against Cadmium Chloride-Induced Oxidative Injury in Goat Sperm and Zygotes. Biological Trace Element Research 2018; 185(2):344-355.(通讯作者, 中科院三区, IF3.9)
11. Hongmei Zhu#, Jun Liu#, Chenchen Cui, Yujie Song, Hengtao Ge, Linyong Hu, Qian Li, Yaping Jin, Yong Zhang. Targeting Human alpha-Lactalbumin Gene Insertion into the Goat beta-Lactoglobulin Locus by TALEN-Mediated Homologous Recombination. PLoS One 2016; 11(6):14.(第一作者, 中科院三区, IF3.7)
12. Chenchen Cui#, Yujie Song#, Jun Liu#, Hengtao Ge, Qian Li, Hui Huang, Linyong Hu, Hongmei Zhu, Yaping Jin, Yong Zhang. Gene targeting by TALEN-induced homologous recombination in goats directs production of beta-lactoglobulin-free, high-human lactoferrin milk. Scientific Reports 2015; 5:10482.(第一作者, 中科院三区, IF4.6)
13. Kun Ru, Feng Su, Yuemao Zheng, Yijun Zhang, Yan Luo, Zekun Guo, Xiaoli He, Xin Liu, Jingcheng Zhang, Jun Liu*, Yong Zhang*. Inducible expression of enhanced green fluorescent protein by interleukin-1 alpha, interleukin-1 beta and Toll-like receptor 2 promoters in goat mammary epithelial cells in response to bacterial challenges. Veterinary Journal 2015; 203(1):85-91.(通讯作者, 中科院二区, IF2.2)
14. Man Zhang, Yuemao Zheng, Wuju Chen, Yan Zhang, Zekun Guo, Yong Zhang*, Jun Liu*. Identifying an optimal promoter sequence of goat beta-lactoglobulin gene for constructing high-expression vectors in mammary epithelial cells. Small Ruminant Research 2015; 131:70-77.(通讯作者, 中科院三区, IF1.8)
15. Jun Liu#, Yongsheng Wang, Jianmin Su, Lijun Wang, Ruizhe Li, Qian Li, Yongyan Wu, Song Hua, Fusheng Quan, Zekun Guo, Yong Zhang*. Effect of the Time Interval Between Fusion and Activation on Epigenetic Reprogramming and Development of Bovine Somatic Cell Nuclear Transfer Embryos. Cellular reprogramming 2013, 15(2):134-142.(第一作者, 中科院四区, IF1.6)
16. Jun Liu#, Yongsheng Wang, Jianmin Su, Yan Luo, Fusheng Quan, Yong Zhang*. Nuclear donor cell lines considerably influence cloning efficiency and the incidence of large offspring syndrome in bovine somatic cell nuclear transfer. Reproduction in Domestic Animals 2013, 48(4):660-664.(第一作者, 中科院三区, IF1.7)
17. Zhongcai Yang#, Jun Liu#, Hongliang Liu, Mingning Qiu, Qingqing Liu, Liming Zheng, Meijun Pang, Fusheng Quan*, Yong Zhang*. Isolation and Characterization of SSEA3+ Stem Cells Derived from Goat Skin Fibroblasts. Cellular Reprogramming 2013, 15(3):195-205. (第一作者, 中科院四区, IF1.6)
18. Yan Luo#, Jun Liu#, Qingqing Liu, Liming Zheng, Yongsheng Wang, Jianmin Su, Guangdong Hu, Yong Zhang*. Chicken hypersensitive site-4 insulator increases human serum albumin expression in bovine mammary epithelial cells modified with phiC31 integrase. Biotechnology letters 2013, 35(4):529-537. (第一作者, 中科院四区, IF2.7)
19. Yan Luo#, Jun Liu#, Yongsheng Wang, Jianmin Su, Yongyan Wu, Guangdong Hu, Mingqing Gao, Fusheng Quan, Yong Zhang*. PhiC31 integrase‐mediated genomic integration and stable gene expression in the mouse mammary gland after gene electrotransfer. Journal of gene medicine 2013, 15(10):356-365. (第一作者, 中科院四区, IF3.5)
20. Jun Liu#, Yan Luo#, Hengtao Ge#, Chengquan Han, Hui Zhang, Yongsheng Wang, Jianmin Su, Fusheng Quan, Mingqing Gao, Yong Zhang*. Anti-bacterial activity of recombinant human β-defensin-3 secreted in the milk of transgenic goats produced by somatic cell nuclear transfer. plosOne 2013, 8(6):e65379. (第一作者, 中科院三区, IF3.7)
21. Jun Liu#, Zhongcai Yang, Mingning Qiu, Yan Luo, Meijun Pang, Yongyan Wu, Yong Zhang*. Developmental Potential of Cloned Goat Embryos from an SSEA3+ Subpopulation of Skin Fibroblasts. Cellular reprogramming 2013, 15(2):159-165.(第一作者, 中科院四区, IF1.6)
22. Jun Liu#, Yan Luo#, Liming Zheng, Qingqing Liu, Zhongcai Yang, Yongsheng Wang, Jianmin Su, Fusheng Quan, Yong Zhang*. Establishment and characterization of fetal fibroblast cell lines for generating human lysozyme transgenic goats by somatic cell nuclear transfer. Transgenic research 2013, 22(5):893-903.(第一作者, 中科院四区, IF3.0)
23. Jun Liu#, Yan Luo, Qingqing Liu, Liming Zheng, Zhongcai Yang, Yongsheng Wang, Jianmin Su, Fusheng Quan, Yong Zhang*. Production of cloned embryos from caprine mammary epithelial cells expressing recombinant human β-defensin-3. Theriogenology 2013, 79(4):660-666. (第一作者, 中科院二区TOP, IF2.8)
24. Jun Liu, Lianglang Li, Shan Du, Xueyao Bai, Hengde Zhang, Shuang Tang, Mingtao Zhao, Fusheng Quan, Xiaoe Zhao, Yong Zhang*. Effects of interval between fusion and activation, cytochalasin B treatment, and number of transferred embryos, on cloning efficiency in goats. Theriogenology 2011, 76(6):1076-1083. (第一作者, 中科院二区TOP, IF2.8)
25. Jun Liu#, Shuang Tang, Wei Xu, Yongsheng Wang, Baoying Yin, Yong Zhang*. Detrimental effects of antibiotics on mouse embryos in chromatin integrity, apoptosis and expression of zygotically activated genes. Zygote 2011, 19(2): 137-145.(第一作者, 中科院四区, IF1.7)
26. 基于供体细胞DNA甲基化水平的修饰提高山羊克隆效率的载体、细胞和方法;发明人:张涌,刘军,韩成全,苏建民,毛廷超,康建;专利授权号ZL20171132 2629.8;2019年授权。
27. 一种牛囊胚内细胞团细胞和滋养层细胞的差异染色方法;发明人:张涌,罗艳,刘军,王勇胜,权富生,郭泽坤,余源;专利授权号ZL201110251106.5;2013年授权;
28. 一种 HSA 乳腺特异性表达载体及其构建的重组细胞;发明人:苏建民,张涌,刘军;专利授权号ZL201210269209.9;2014年授权。
五、科研项目
1. 国家重大专项,优质高产抗病乳用羊新品种设计与培育,2022.12-2025.12,8698万元,主持人;
2. 国家自然科学基金面上项目,内源性逆转录病毒元件转录激活调控山羊早期胚胎发育的功能研究,2018.01-2021.12,61万元,主持人;
3. 国家自然科学基金面上项目,内源性逆转录病毒ERV1元件编码的Gag蛋白调控山羊早期胚胎发育的功能和机制,2021.1-2024.12,58万元,主持人;
4. 国家自然科学基金青年项目,Tet3蛋白在山羊早期胚胎DNA甲基化重编程过程中的作用,2014.1-2016.12,25万元,主持人;
5. 陕西省重点研发计划,利用乳腺生物反应器技术表达猪蓝耳病病毒重要结构蛋白,2015.1-2017.12,20万元,主持人;
6. 内蒙古自治区科技计划项目,种公牛克隆繁育技术研究,2021.1-2023.12, 30万元,主持人;
7. 国家种质资源库项目,羊种质资源冻精胚胎抢救性收集,2022.10-2023.10,30万元,主持人;
8. 企业横向课题,山羊良种繁育技术研究与应用,2020.1-2021.12,20万元,主持人;
9. 国家重大专项,抗病转基因牛新品种培育,2016.01-2020.12,3688.93万元,项目骨干。
六、荣誉及奖项
1. 2016年获国家技术发明二等奖“良种牛羊高效克隆技术”,成果完成人排名第六。
2. 2015年获陕西省科技进步一等奖“牛羊基因定点精确编辑技术研究与应用”一项,成果完成人排名第五。
3. 2013年获陕西省科技进步一等奖“牛羊良种繁育关键技术研究与应用”一项,成果完成人排名第三。
七、联系方式
通讯地址:陕西省咸阳市杨凌区西北农林科技大学南校区家畜生物学实验室,邮编712100
联系电话:029-87080092 Email: liujun2013@nwsuaf.edu.cn或liuluo1028@163.com